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pcaggs vector  (New England Biolabs)


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    Structured Review

    New England Biolabs pcaggs vector
    Pcaggs Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 15375 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcaggs+vector/EcoRI/pmc13059714-228-13-20
    Average 99 stars, based on 15375 article reviews
    pcaggs vector - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Metagenomics-enabled reverse-genetics assembly and characterization of myotis bat morbillivirus.
    Article Snippet: CHO cells were grown in DMEM/F12 (1:1) medium (Gibco) with 10% FBS. .. We cloned the ORF of hCD150, dCD150 and bCD150 (from Myostis brandtii since the CD150 sequence from M. riparius is unknown) into the pCAGGS vector cut by EcoRI (NEB) and NheI-HF (NEB). ..

    Article Title: Conserved Functions of Orthohepadnavirus X Proteins to Inhibit Type-I Interferon Signaling
    Article Snippet: .. Inserts encoding cDNA were cloned into the pCAGGS vector [ ], predigested with EcoRI-HF (New England Biolabs [NEB], Ipswich, MA, USA, Cat# R3101M) and NheI-HF (NEB, Cat# R3131M) using In-Fusion Snap Assembly Master Mix (TaKaRa, Kusatsu, Japan, Cat# Z8947N). .. Plasmids were amplified using NEB 5-alpha F Iq Competent E. coli (High Efficiency) (NEB, Cat# C2992H) and extracted with PureYield Plasmid Miniprep System (Promega, Cat# A1222).

    Article Title: Metagenomics-enabled reverse-genetics assembly and characterization of myotis bat morbillivirus
    Article Snippet: CHO cells were grown in DMEM/F12 (1:1) medium (gibco) with 10% FBS. .. We cloned the open reading frame of hCD150, dCD150, and bCD150 (from Myostis brandtii since the CD150 sequence from M. riparius is unknown) into the pCAGGS vector cut by Eco RI (NEB) and Nhe I-HF (NEB). .. NCI-H441 cells (ATCC Ca# HTB-174) were grown in RPMI 1640 medium (ThermoFisher Scientific, USA) with 10% FBS.

    Article Title: The EP424R protein of African swine fever virus functions as a 2'-O-methyltransferase and plays an important role in viral replication.
    Article Snippet: The pET42b vector was digested with Nde I and Xho I (NEB) with eight histidine residues in the C-terminus. .. For eukaryotic expression, the EP424R fragment was cloned and inserted into a linearized pCAGGS vector (EcoRI and XhoI restriction sites; NEB) through homologous recombination. .. The pCAGGS vector was digested with EcoRI and XhoI (NEB) with an HA-tag at the N-terminus.

    Article Title: Human herpesvirus 6B glycoprotein B postfusion structure, vulnerability mapping, and receptor recognition.
    Article Snippet: .. The wild-type HHV-6B gB (residues 1–680, tagged with 6*His or Flag) plasmid was used as the template, and the amplified fragments were then cloned and inserted into a pCAGGS vector digested with EcoRI (NEB, CAT#R3101V) and MluI (NEB, CAT#R3198V) via the ClonExpress Ultra One Step Cloning Kit V2 (Vazyme, CAT#C116-02). ..

    Article Title: The EP424R protein of African swine fever virus functions as a 2′-O-methyltransferase and plays an important role in viral replication
    Article Snippet: The pET42b vector was digested with Nde I and Xho I (NEB) with eight histidine residues in the C-terminus. .. For eukaryotic expression, the EP424R fragment was cloned and inserted into a linearized pCAGGS vector (EcoRI and XhoI restriction sites; NEB) through homologous recombination. .. The pCAGGS vector was digested with EcoRI and XhoI (NEB) with an HA-tag at the N-terminus.

    Article Title: Human herpesvirus 6B glycoprotein B postfusion structure, vulnerability mapping, and receptor recognition
    Article Snippet: .. The wild-type HHV-6B gB (residues 1–680, tagged with 6*His or Flag) plasmid was used as the template, and the amplified fragments were then cloned and inserted into a pCAGGS vector digested with EcoRI (NEB, CAT#R3101V) and MluI (NEB, CAT#R3198V) via the ClonExpress Ultra One Step Cloning Kit V2 (Vazyme, CAT#C116-02). ..

    Article Title: Conserved use of the sodium/bile acid cotransporter (NTCP) as an entry receptor by hepatitis B virus and domestic cat hepadnavirus.
    Article Snippet: .. For transient expression of NTCP, inserts encoding NTCP cDNA were cloned into a pCAGGS vector (Niwa et al., 1991) predigested with EcoRI-HF (NEB, Cat# R3101M) and NheI-HF (NEB, Cat# R3131M) using In-Fusion Snap Assembly Master Mix (TaKaRa, Cat# Z8947N). .. In addition, for stable expression of NTCP, inserts encoding NTCP cDNA of human, cat, and cynomolgus monkey were cloned into a pLVSIN-CMV Hyg vector (TaKaRa, Cat# 6182) predigested with NotI-HF (NEB, Cat# R3189L) and BamHI-HF (NEB, Cat# R3136L) using In-Fusion Snap Assembly Master Mix.

    Sequencing:

    Article Title: Metagenomics-enabled reverse-genetics assembly and characterization of myotis bat morbillivirus.
    Article Snippet: CHO cells were grown in DMEM/F12 (1:1) medium (Gibco) with 10% FBS. .. We cloned the ORF of hCD150, dCD150 and bCD150 (from Myostis brandtii since the CD150 sequence from M. riparius is unknown) into the pCAGGS vector cut by EcoRI (NEB) and NheI-HF (NEB). ..

    Article Title: Metagenomics-enabled reverse-genetics assembly and characterization of myotis bat morbillivirus
    Article Snippet: CHO cells were grown in DMEM/F12 (1:1) medium (gibco) with 10% FBS. .. We cloned the open reading frame of hCD150, dCD150, and bCD150 (from Myostis brandtii since the CD150 sequence from M. riparius is unknown) into the pCAGGS vector cut by Eco RI (NEB) and Nhe I-HF (NEB). .. NCI-H441 cells (ATCC Ca# HTB-174) were grown in RPMI 1640 medium (ThermoFisher Scientific, USA) with 10% FBS.

    Plasmid Preparation:

    Article Title: Metagenomics-enabled reverse-genetics assembly and characterization of myotis bat morbillivirus.
    Article Snippet: CHO cells were grown in DMEM/F12 (1:1) medium (Gibco) with 10% FBS. .. We cloned the ORF of hCD150, dCD150 and bCD150 (from Myostis brandtii since the CD150 sequence from M. riparius is unknown) into the pCAGGS vector cut by EcoRI (NEB) and NheI-HF (NEB). ..

    Article Title: Metagenomics-enabled reverse-genetics assembly and characterization of myotis bat morbillivirus
    Article Snippet: CHO cells were grown in DMEM/F12 (1:1) medium (gibco) with 10% FBS. .. We cloned the open reading frame of hCD150, dCD150, and bCD150 (from Myostis brandtii since the CD150 sequence from M. riparius is unknown) into the pCAGGS vector cut by Eco RI (NEB) and Nhe I-HF (NEB). .. NCI-H441 cells (ATCC Ca# HTB-174) were grown in RPMI 1640 medium (ThermoFisher Scientific, USA) with 10% FBS.

    Article Title: The EP424R protein of African swine fever virus functions as a 2'-O-methyltransferase and plays an important role in viral replication.
    Article Snippet: The pET42b vector was digested with Nde I and Xho I (NEB) with eight histidine residues in the C-terminus. .. For eukaryotic expression, the EP424R fragment was cloned and inserted into a linearized pCAGGS vector (EcoRI and XhoI restriction sites; NEB) through homologous recombination. .. The pCAGGS vector was digested with EcoRI and XhoI (NEB) with an HA-tag at the N-terminus.

    Article Title: Human herpesvirus 6B glycoprotein B postfusion structure, vulnerability mapping, and receptor recognition.
    Article Snippet: .. The wild-type HHV-6B gB (residues 1–680, tagged with 6*His or Flag) plasmid was used as the template, and the amplified fragments were then cloned and inserted into a pCAGGS vector digested with EcoRI (NEB, CAT#R3101V) and MluI (NEB, CAT#R3198V) via the ClonExpress Ultra One Step Cloning Kit V2 (Vazyme, CAT#C116-02). ..

    Article Title: The EP424R protein of African swine fever virus functions as a 2′-O-methyltransferase and plays an important role in viral replication
    Article Snippet: The pET42b vector was digested with Nde I and Xho I (NEB) with eight histidine residues in the C-terminus. .. For eukaryotic expression, the EP424R fragment was cloned and inserted into a linearized pCAGGS vector (EcoRI and XhoI restriction sites; NEB) through homologous recombination. .. The pCAGGS vector was digested with EcoRI and XhoI (NEB) with an HA-tag at the N-terminus.

    Article Title: Human herpesvirus 6B glycoprotein B postfusion structure, vulnerability mapping, and receptor recognition
    Article Snippet: .. The wild-type HHV-6B gB (residues 1–680, tagged with 6*His or Flag) plasmid was used as the template, and the amplified fragments were then cloned and inserted into a pCAGGS vector digested with EcoRI (NEB, CAT#R3101V) and MluI (NEB, CAT#R3198V) via the ClonExpress Ultra One Step Cloning Kit V2 (Vazyme, CAT#C116-02). ..

    Article Title: Conserved use of the sodium/bile acid cotransporter (NTCP) as an entry receptor by hepatitis B virus and domestic cat hepadnavirus.
    Article Snippet: .. For transient expression of NTCP, inserts encoding NTCP cDNA were cloned into a pCAGGS vector (Niwa et al., 1991) predigested with EcoRI-HF (NEB, Cat# R3101M) and NheI-HF (NEB, Cat# R3131M) using In-Fusion Snap Assembly Master Mix (TaKaRa, Cat# Z8947N). .. In addition, for stable expression of NTCP, inserts encoding NTCP cDNA of human, cat, and cynomolgus monkey were cloned into a pLVSIN-CMV Hyg vector (TaKaRa, Cat# 6182) predigested with NotI-HF (NEB, Cat# R3189L) and BamHI-HF (NEB, Cat# R3136L) using In-Fusion Snap Assembly Master Mix.

    Expressing:

    Article Title: The EP424R protein of African swine fever virus functions as a 2'-O-methyltransferase and plays an important role in viral replication.
    Article Snippet: The pET42b vector was digested with Nde I and Xho I (NEB) with eight histidine residues in the C-terminus. .. For eukaryotic expression, the EP424R fragment was cloned and inserted into a linearized pCAGGS vector (EcoRI and XhoI restriction sites; NEB) through homologous recombination. .. The pCAGGS vector was digested with EcoRI and XhoI (NEB) with an HA-tag at the N-terminus.

    Article Title: The EP424R protein of African swine fever virus functions as a 2′-O-methyltransferase and plays an important role in viral replication
    Article Snippet: The pET42b vector was digested with Nde I and Xho I (NEB) with eight histidine residues in the C-terminus. .. For eukaryotic expression, the EP424R fragment was cloned and inserted into a linearized pCAGGS vector (EcoRI and XhoI restriction sites; NEB) through homologous recombination. .. The pCAGGS vector was digested with EcoRI and XhoI (NEB) with an HA-tag at the N-terminus.

    Article Title: Conserved use of the sodium/bile acid cotransporter (NTCP) as an entry receptor by hepatitis B virus and domestic cat hepadnavirus.
    Article Snippet: .. For transient expression of NTCP, inserts encoding NTCP cDNA were cloned into a pCAGGS vector (Niwa et al., 1991) predigested with EcoRI-HF (NEB, Cat# R3101M) and NheI-HF (NEB, Cat# R3131M) using In-Fusion Snap Assembly Master Mix (TaKaRa, Cat# Z8947N). .. In addition, for stable expression of NTCP, inserts encoding NTCP cDNA of human, cat, and cynomolgus monkey were cloned into a pLVSIN-CMV Hyg vector (TaKaRa, Cat# 6182) predigested with NotI-HF (NEB, Cat# R3189L) and BamHI-HF (NEB, Cat# R3136L) using In-Fusion Snap Assembly Master Mix.

    Homologous Recombination:

    Article Title: The EP424R protein of African swine fever virus functions as a 2'-O-methyltransferase and plays an important role in viral replication.
    Article Snippet: The pET42b vector was digested with Nde I and Xho I (NEB) with eight histidine residues in the C-terminus. .. For eukaryotic expression, the EP424R fragment was cloned and inserted into a linearized pCAGGS vector (EcoRI and XhoI restriction sites; NEB) through homologous recombination. .. The pCAGGS vector was digested with EcoRI and XhoI (NEB) with an HA-tag at the N-terminus.

    Article Title: The EP424R protein of African swine fever virus functions as a 2′-O-methyltransferase and plays an important role in viral replication
    Article Snippet: The pET42b vector was digested with Nde I and Xho I (NEB) with eight histidine residues in the C-terminus. .. For eukaryotic expression, the EP424R fragment was cloned and inserted into a linearized pCAGGS vector (EcoRI and XhoI restriction sites; NEB) through homologous recombination. .. The pCAGGS vector was digested with EcoRI and XhoI (NEB) with an HA-tag at the N-terminus.

    Amplification:

    Article Title: Human herpesvirus 6B glycoprotein B postfusion structure, vulnerability mapping, and receptor recognition.
    Article Snippet: .. The wild-type HHV-6B gB (residues 1–680, tagged with 6*His or Flag) plasmid was used as the template, and the amplified fragments were then cloned and inserted into a pCAGGS vector digested with EcoRI (NEB, CAT#R3101V) and MluI (NEB, CAT#R3198V) via the ClonExpress Ultra One Step Cloning Kit V2 (Vazyme, CAT#C116-02). ..

    Article Title: Human herpesvirus 6B glycoprotein B postfusion structure, vulnerability mapping, and receptor recognition
    Article Snippet: .. The wild-type HHV-6B gB (residues 1–680, tagged with 6*His or Flag) plasmid was used as the template, and the amplified fragments were then cloned and inserted into a pCAGGS vector digested with EcoRI (NEB, CAT#R3101V) and MluI (NEB, CAT#R3198V) via the ClonExpress Ultra One Step Cloning Kit V2 (Vazyme, CAT#C116-02). ..

    Cloning:

    Article Title: Human herpesvirus 6B glycoprotein B postfusion structure, vulnerability mapping, and receptor recognition.
    Article Snippet: .. The wild-type HHV-6B gB (residues 1–680, tagged with 6*His or Flag) plasmid was used as the template, and the amplified fragments were then cloned and inserted into a pCAGGS vector digested with EcoRI (NEB, CAT#R3101V) and MluI (NEB, CAT#R3198V) via the ClonExpress Ultra One Step Cloning Kit V2 (Vazyme, CAT#C116-02). ..

    Article Title: Human herpesvirus 6B glycoprotein B postfusion structure, vulnerability mapping, and receptor recognition
    Article Snippet: .. The wild-type HHV-6B gB (residues 1–680, tagged with 6*His or Flag) plasmid was used as the template, and the amplified fragments were then cloned and inserted into a pCAGGS vector digested with EcoRI (NEB, CAT#R3101V) and MluI (NEB, CAT#R3198V) via the ClonExpress Ultra One Step Cloning Kit V2 (Vazyme, CAT#C116-02). ..



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